strategy
Queen's University Protein Function Discovery
and Department of Biomedical and Molecular Sciences
Molecular Modelling and Crystallographic Computing Facility
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  • Getting started with the data collection Strategy program


    Strategy Data collection strategy optimization
    (IRIX only)
    This program by Raimond Ravelli1 can be used to optimize the oscillation range used to collect data on the two R-Axis image plate systems. The program can be run either interactively, using the GUI interface or from a file of commands. To run this program:
    1. set up the operating environment and add the necessary directories to your path:
      source /prog/setup strategy

    2. collect one oscillation frame

    3. autoindex and integrate the frame with denzo to give a file of integrated intensities (a ".x" file in the denzo nomenclature). Note that one can also ask strategy to read a scalepack output file and then calculate how to collect the data that is missing (use the "ALREADY MEASURED file.sca" command).

    To use the command file mode: copy one of the example input files from /prog/strategy/examples (or see the examples shown below) and edit to change the file names, space group and detector name if necessary. Then do

    strategy < strat.inp > strat.log

    where the input file, strat.inp, could look like one of the following:

    R-Axis II:
    	XFILE crystal.x
    	NBINS 10
    	PSFILE strategy.ps
    	FORMAT RAXVERTICAL
    	SPACE GROUP c2
    	ALREADY MEASURED crystal.sca
    	
    R-Axis IV:
    	XFILE crystal.x
    	NBINS 10
    	PSFILE strategy.ps
    	format RAXISIV
    	space group p212121
    	

    strat_GUI To use the GUI interface mode do

    StratX file.x

    where file.x is the file of your integrated intensities. You will be presented with an input box, where you can select anomalous and leapfrog options. If you click on leapfrog then the box will enlarge to the allow the entry of various sweep possibilities as shown at right. (The GUI is not described in the manual at all, as it was created by John Skinner at NSLS beamline X-12C.)

    strategy postscript output While the GUI mode is easier to run, it provides somewhat less information as the full output of strategy is only stripped to give the list of starting and sweep angles, and the completeness. The full output also gives estimates of the reduncancy in a format identical to the scalepack output. The GUI mode will create a strat.inp file to allow you to rerun it in command file mode and to save the output to a file. Both modes produce a postscript file that contains several pages of polar diagrams for the best runs that strategy has been able to devise. The plots is made up of many radial lines (in red) whose length represents the sweep angle and whose angle from the phi=0 axis represents the sweep starting position. Thus the points on the perimeter of the resulting red shape that are closest to the origin represent sweep starting positions for which one needs the shortest sweep to get the desired completeness.



    1Ravelli RBG, Sweet RM, Skinner JM, Duisenberg AJM and Kroon J "STRATEGY: a program to optimize the starting angle and scan range for X-ray data collection" J. Appl. Cryst. (1997) 30, 551-554. See also the local copy of the manual for more information and the Strategy Home Page for further information.
    Last revised: Tuesday, 16-Sep-2003 10:17:50 EDT